Evaluation of Lactic Acid Bacteria Isolated from Piglets Tract and Encapsulation of Selected Probiotic Cells

Fourteen lactic acid bacteria (LAB) strains were isolated from the intestinal tract and feces of piglets. Among these isolates, only twelve biochemically (API 50 CHL ABIS online for bacterial identification) genetically (16S rRNA sequencing) confirmed as Limosilactobacillus fermentum, Lactobacillus acidophilus, Lactiplantibacillus plantarum. Experiments to evaluate probiotic potential isolates including pH tolerance (pH 2.0 3.0), bile salts (0.3% ox gall) resistance, hemolysis activity, antibiotic susceptibility, high-temperature resistance tested. Only two identified exhibited high survival rates when exposed low salts, L. acidophilus IBNA 76 plantarum 84. The test presented 100% both gentamicin, kanamycin, lincomycin, colistin sulfate, erythromycin, amikacin, oxytetracycline, enrofloxacin, streptomycin, tilmicosin, lower than 0.6 mm inhibition zone diameter. Promising (L. 84) spray-drying technique based on visible rates. Carrier matrix material was used a maltodextrin-glucose solution. encapsulation survived over 67% 77%, corresponding decrease in strain viability 109 107 CFU/g. After further vitro evaluations, findings this study showed that, all LAB strains, 84 may be considered candidates animal nutrition have promising performance piglet feed due their origin isolation.